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Is there a way to tell for sure if an egg is fertilized before putting it in the incubator?

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The oldest and easiest way to tell if an egg is fertilized is called candling the egg. It is literally holding the egg up to a lit candle {not to warm it, but in order to see inside of the egg}. You can also use a very bright small flashlight. If the egg appears opaque, it is probably a fertilized egg